Dr David Brough

David Brough – Research summary

Dr David Brough

Dr David Brough

I am a lecturer in the Faculty of Life Sciences and work within both the Immunology and Neuroscience research sections. My primary research focus is to understand the regulation of the processing and secretion of the ‘master’ cytokine interleukin-1 (IL-1). IL-1 is regulated by pattern recognition receptors on macrophages during infection or after injury. Of particular interest is how a subset of pattern recognition receptors form inflammasomes after stimulation that assemble in to large complexes that activate the enzyme caspase-1. Caspase-1 cleaves a form of IL-1 (IL-1beta) from an inactive precursor to a mature secreted form. One of my main research goals is to understand the regulation of this process. How IL-1 is secreted from cells is also unknown and represents a major open question in inflammation research and is also central to my research focus. IL-1-driven inflammation worsens central nervous system disease and I aim to translate my fundamental discoveries in to disease models of stroke, Alzheimer’s and epilepsy. Ultimately I hope that the fundamental biology performed in my lab has a translational impact.

People

David Brough – People

 

Alex Baldwin (With Dr Sally Freeman FMHS)
Dr Hazel Ball
Catherine Diamond (with Dr Alessandra Mortellaro, ASTAR Singapore)
Michelle Edye (with Prof Stuart Allan)
Dr Paul Maclellan (with Dr Sally Freeman FMHS)
Dean Plumbley (With Prof Pedro Mendes, CS)
Jordie Roberts
Catherine Savage
Jennifer Smith (With Prof Hugh Piggins)

People

David Brough – People

 

Alex Baldwin (With Dr Sally Freeman FMHS)
Dr Hazel Ball
Catherine Diamond (with Dr Alessandra Mortellaro, ASTAR Singapore)
Michelle Edye (with Prof Stuart Allan)
Dr Paul Maclellan (with Dr Sally Freeman FMHS)
Dean Plumbley (With Prof Pedro Mendes, CS)
Jordie Roberts
Catherine Savage
Jennifer Smith (With Prof Hugh Piggins)

Collaborators

Prof Pedro Mendes (CS)
Dr Josh Knowles (CS)
Dr Sally Freeman (FMHS)
Prof Stuart Allan
Prof Nancy Rothwell
Prof Richard Baines
Prof Ian Roberts
Dr Lisa Swanton
Prof Stephen High
Dr Adrian Parry-Jones (FMHS)
Dr James Galea (FMHS)
Dr Pablo Pelegrin (Murcia Health Foundation, Spain)
Dr Igor Prudovsky (Maine Medical Center Research Institute, US)
Dr Alessandro Mortellaro (AStar Immunology, Singapore)
(Industrial links with Johnson and Johnson, Brickell Biotech)

Publications

  • Brough D, Rothwell NJ. (2007). Caspase-1-dependent processing of pro-interleukin-1beta is cytosolic and precedes cell death. The Journal of Cell Science, 120, 772-781. eScholarID:1c6261 | DOI:10.1242/jcs.03377
  • Luheshi NM, Rothwell NJ, Brough D. (2009). The dynamics and mechanisms of interleukin-1alpha and Beta nuclear import. Traffic (Copenhagen, Denmark), 10(1), 16-25. eScholarID:1c8176 | PMID:18939951 | DOI:10.1111/j.1600-0854.2008.00840.x
  • Brough D, Pelegrin P, Rothwell NJ. (2009). Pannexin-1-dependent caspase-1 activation and secretion of IL-1beta is regulated by zinc. European journal of immunology, 39(2), 352-8. eScholarID:1c8177 | PMID:19130485 | DOI:10.1002/eji.200838843
  • Luheshi NM, McColl BW, Brough D. (2009). Nuclear retention of IL-1alpha by necrotic cells: A mechanism to dampen sterile inflammation. European Journal of Immunology, 39, 2973-2980. eScholarID:75588 | DOI:10.1002/eji.200939712