Professor David Thornton

Dave Thornton research summary

David Thornton

David Thornton

The research in my laboratory is focused on understanding how the gel-like substance mucus protects the body. This critically important innate defence barrier protects the delicate mucosal surfaces of the body against attack from a range of external agents (e.g. noxious gases, toxic substances and pathogens).

Our research is currently focused on the complex interplay between the mucus barrier, the immune system and infectious challenges (bacterial, fungal and parasitic worms).We are actively investigating the role of the mucus barrier and its mucin components in protection against gastrointestinal (and lung) helminth infection. The mucus barrier is an important part of a well-coordinated response to expel gut-living worms and we are focusing on the immunoregulatory effects of infectious challenge on barrier composition, structure and function.

In a new development, we are employing the Xenopus tropicalis tadpole skin surface to investigate molecular mechanisms of innate defence.  We are probing the defensive capabilities of the tadpole surface mucus barrier to uncover general principles in innate defence.

Email: dave.thornton@manchester.ac.uk | Tel: 0161 275 5647

People

Dave Thornton people

Dr Ajanta Chakraborty
Dr Eamon Dubaissi
Dr Caroline Ridley
Abigail Cowley
Evelyn Funjika
Gareth Hughes
Elena Owen
Catherine Sharpe
Rebecca Shears

People

Dave Thornton people

Dr Ajanta Chakraborty
Dr Eamon Dubaissi
Dr Caroline Ridley
Abigail Cowley
Evelyn Funjika
Gareth Hughes
Elena Owen
Catherine Sharpe
Rebecca Shears

Publications

  • Hasnain, S.Z., Thornton, D.J and Grencis, R.J. (2011) Changes in the mucosal barrier during acute and chronic Trichuris muris infection. Parasite Immunology 33(1): 45-55. PMID 21155842
  • Hasnain, S.Z., Evans, C.M., Roy, M., Gallagher, A.L., Kindrachuk, K.N., Barron, L., Dickey, B.F., Wilson, M.S., Wynn, T.A., Grencis, R.K. and Thornton, D.J. (2011) Muc5ac: A critical component mediating the rejection of the eneteric nematode, Trichuris muris. J. Exp. Med. 208(5), 893-900. PMID 21502330
  • Barbier, D., Garcia-Verdugo, I., Pothlichet, J., Khazen, R., Descamps, D., Rousseau, K., Thornton, D., Si-Tahar, M., Touqui, L., Chignard, M. & Sallenave, J-M. (2012) Influenza A induces the major secreted airway mucin MUC5AC in a protease-EGFR-ERK-Sp1 dependent pathway. Am. J. Resp. Cell & Mol. Biol. 47(2), 149-57.PMID 22383584
  • Farnell, E., Rousseau, K., Thornton, D. J., Bowyer, P. and Herrick, S. (2012) Expression and secretion of Aspergillus fumigatus proteases is regulated in response to different protein substrates. Fungal Biol. 116(9), 1003-12. PMID 22954343
  • Hasnain, S., Grencis, R.K. and Thornton, D.J. (2012) Serine protease(s) secreted by the nematode Trichuris muris degrade the mucus barrier. PLoS Neg. Trop. Dis. 6(10), e1856 PMID 23071854
  • Hasnain, S.Z., Gallagher, A. L., Grencis, R.K. and Thornton D.J. (2013) A new role for mucins in immunity; insights gained from gastrointestinal nematode infection. Int. J. Biochem. Cell Biol. 45, 364-374. PMID 23107603
  • Roy, M.G., Livraghi-Butrico, A., Fletcher, A.A., McElwee, M.M., Evans, S.E., Boerner, R.M., Alexander, S.N., Bellinghausen, L.K., Song, A.S., Petrova, Y.M., Tuvim, M.J., Adachi, R., Romo1, I., Bordt, A.S., Bowden, M.G., Sisson, J.H., Woodruff, P.G., Thornton, D.J., Rousseau, K., De la Garza, M.M., Moghaddam, S.J., Karmouty-Quintana, H., Blackburn, M.R., Drouin, S.M., Davis, C.W., Terrell, K.A., Grubb, B.R., O’Neal, W.K., Flores, S.C., Cota-Gomez, A., Lozupone, C.A., Donnelly, J.A., Watson, A.M., Hennessy, C.E., Keith, R.C., Yang, I.V., Barthel, L., Henson, P.M., Janssen, W.J., Schwartz, D.A., Boucher, R.C., Dickey, B.F. and Evans, C.M. (2014) Muc5b is required for airway defence. Nature, 505 (7483), 412-6. PMID 24317696
  • Dubaissi, E., Rousseau, K., Lea, R., Soto, X., Nardeosingh, S., Amaya, E., Thornton D.J. and Papalopulu, P. (2014) A secretory cell type develops alongside ionocytes, goblet and multiciliated cells and provides a protective, anti-infective, function in the frog embryonic mucociliary epidermis. Development, 141 (7), 1514-25. PMID 24598166
  • Ridley, C., Kouvatsos, N., Raynal, B.D., Howard, M., Collins R.F., Desseyn J.L., Jowitt T.A., Baldock C., Davis C.W., Hardingham T.E. and Thornton DJ. (2014) Assembly of the Respiratory Mucin MUC5B: A NEW MODEL FOR A GEL-FORMING MUCIN. J Biol Chem. 289 (23), 16409-20. PMID 24778189
  • Wang, H., Kim, J., Denou, E., Gallagher, A., Thornton, D.J., Shajib, Md., Xia, L., Schertzer, J., Grencis, R., Philpott, D. and Khan, W. (2016) A new role of Nod proteins in regulation of intestinal goblet cell response in the context of innate host defense in an enteric parasite infection. Infection and Immunity, 84, 275-285. PMID 26527214
  • Cowley, A., Thornton, D.J., Denning, D.W. and Horsley, A. (2016) Aspergillosis and the role of mucins in cystic fibrosis. Ped. Pulmonol. PMID 27870227